Fluorescence polarization assay, competitive enzyme-linked immunosorbent assay (ELISA-C) and indirect ELISA for the diagnosis of brucellosis in buffaloes (Bubalus bubalis)
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Immunodiagnosis of Haemonchus contortus infection in sheep by indirect enzyme linked immunosorbent assay (ELISA)
Indirect plate enzyme-linked immunosorbent assay was standardized and evaluated for its effectivenessin immunodiagnosis of haemonchosis in experimental and clinical cases in sheep by using somatic wholeadult antigen of H. contortus. Plate ELISA was standardized using 5 μg/well antigen concentration with1:100 and 1:1000 of sera and conjugate dilution. Indirect plate ELISA was able to demonstrate...
متن کاملAn enzyme-linked immunosorbent assay (ELISA) to measure growth hormone level in serum and milk of buffaloes (Bubalus bubalis).
An indirect Sandwich ELISA to measure growth hormone level in serum and milk of buffaloes was developed. The assay was based on purified anti rbST IgG raised in rabbits and chicken and rabbit anti chicken IgG horseradish peroxidase. The assay was validated in terms of sensitivity, specificity, precision and recovery. Parallelism was demonstrated between the standard curve and serially diluted s...
متن کاملThe ELISA, enzyme-linked immunosorbent assay.
Featured Article: Engvall E, Perlmann P. Enzymelinked immunosorbent assay (ELISA). Quantitative assay of immunoglobulin G. Immunochemistry 1971;8:871– 4. This paper was my first as a graduate student of Professor Peter Perlmann at Stockholm University. At the time, RIAs were in full bloom, but they were too sophisticated for many areas of research and diagnosis because they required expensive e...
متن کاملIndirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes
R. Munir, S. T. ur Rehman, R. Kausar, S. M. Saqlain Naqvi, U. Farooq: Indirect Enzyme Linked Immunosorbent Assay for Diagnosis of Brucellosis in Buffaloes. Acta Vet. Brno 2008, 77: 401-406. Brucellosis is an important zoonotic disease causing significant economic losses worldwide. Early detection of this disease is essential for its control and eradication. Presently, an Indirect Enzyme Linked ...
متن کاملDevelopment of an enzyme - linked immunosorbent assay ( ELISA ) for the
6 Department of Parasitology and Parasitic Diseases, Faculty of Veterinary Medicine, University of 7 Agricultural Sciences and Veterinary Medicine Cluj-Napoca, Cluj-Napoca, Romania; Veterinary 8 Mycology, Department of Infectious and Parasitic Diseases, Faculty of Veterinary Medicine, 9 University of Liège, Liège, Belgium; Dermatology and Venereology Department, Laboratory of 10 Mycology, Centr...
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ژورنال
عنوان ژورنال: Ciência Rural
سال: 2012
ISSN: 1678-4596
DOI: 10.1590/s0103-84782012005000070